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Fig. 4. Pax3 is necessary and sufficient for CXCL14 expression in the hatching gland. (A)
Embryos injected with Pax3 morpholino (Pax3MO; 40ng) exhibit a strong reduction of CXCL14 expression in the hatching gland (arrow). In these Pax3-depleted embryos Snail2 and Xhe expression is also reduced at the neurula and tailbud stages, respectively (arrows). (B) Pax3 overexpression (0.5 ng) in the whole embryo results in a dramatic expansion of CXCL14 and Xhe expression domains at stage 23 (arrows), while Snail2 expression is reduced at stage 15 (arrow). For the embryos hybridized with CXCL14 or Xhe, anterior view is shown, dorsal to top. For Snail2 the embryos are viewed from the dorsal side anterior to top. (C) In animal explants CXCL14 and Xhe are activated by higher doses of Pax3GR mRNA as compared to Snail2. The concentrations of injected Pax3GR mRNA are 0.05 ng, 0.1 ng, 0.25 ng, 0.5 ng and 1ng. Each Real-Time RT-PCR value has been normalized to the level of EF- 1α expression. |