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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
zic3
|
|
laevis
|
NF stage 9
|
marginal zone
|
zic3
|
|
laevis
|
NF stage 10.5
to
NF stage 12
|
neuroectoderm
,
involuting marginal zone
,
involuted dorsal mesoderm
,
involuted ventral mesoderm
,
non-involuting marginal zone
|
zic3
|
|
laevis
|
NF stage 14
|
chordal neural plate
|
zic1
|
|
laevis
|
NF stage 9
|
marginal zone
,
endomesoderm
|
zic1
|
|
laevis
|
NF stage 10.5
to
NF stage 11
|
ectoderm
,
non-involuting marginal zone
|
zic1
|
|
laevis
|
NF stage 12
|
ectoderm
,
neuroectoderm
,
mesoderm
,
involuted dorsal mesoderm
,
dorsal
|
zic1
|
|
laevis
|
NF stage 14
|
pre-chordal neural plate
,
mesoderm
,
dorsal
,
anterior neural fold
,
anterior
|
zic2
|
|
laevis
|
NF stage 9
|
animal cap
,
marginal zone
,
endomesoderm
|
zic2
|
|
laevis
|
NF stage 10.5
|
involuted dorsal mesoderm
|
zic2
|
|
laevis
|
NF stage 11
|
ectoderm
,
dorsal marginal zone
,
upper blastopore lip
,
dorsal
|
zic2
|
|
laevis
|
NF stage 12
|
ectoderm
,
neuroectoderm
,
mesoderm
,
non-involuting marginal zone
|
zic2
|
|
laevis
|
NF stage 14
|
neuroectoderm
,
head endoderm
,
gastrocoel roof plate
,
anterior neural fold
,
suprablastoporal endoderm
|
|
|
Supplemental figure 1. Expression profiles of Zic and β-catenin from early gastrula to neurula stages Spatial expression patterns of Xenopus Zic1, Zic2 and Zic3 were examined with whole-mount in situ hybridization analysis with a series of cut embryos at various stages from late blastula [Stages (St.) 9.5-9.75] to neurula (St.14). The three Zic genes were chosen for the analysis because they were expressed strongly during gastrulation (Supplemental figure 2). The animal pole is up and dorsal is right. At early gastrula stages, the expression of Zic genes was observed in the dorsal mesoderm area. Zic1, Zic2, and Zic3 expression was observed in the dorsal involuting mesoderm area, as well as in neuroectoderm, throughout the gastrula stages (St. 10.5, 11, 12). Zic3 expression was clearly observed in whole involuting mesoderm surrounding yolk plug, as well as previously reported (Kitaguchi et al., 2000). Zic expression in gastrula mesoderm mostly overlapped with that of β-catenin. |
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ctnnb1 (catenin beta 1) gene expression in bisected Xenopus laevis embryo, NF stage 10.5, assayed by in situ hybridization, dorsal left
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Fig. 6. β-catenin nuclear localization is impaired by Zic3 expression in gastrula embryos. (A) Representative images for β-catenin immunofluorescence staining. Neighboring sections were stained with anti-β-catenin and anti-β-galactosidase (β-gal) antibodies. In each image, the animal pole is at the top and the dorsal side is to the right. Upper images show the β-catenin signal (nuclear, green; non-nuclear, blue) and DAPI signal (nuclear, red), and lower images show the β-galactosidase signal (gray) of the injected area. The yellow indicates the overlapping of nuclear β-catenin and DAPI signals. The insets are low magnifications in which the boxed regions indicate the stained regions. (B) The graph shows percentages of the β-catenin/DAPI double-positive cell nuclei area among the total DAPI-stained cell nuclei in the organizer region. Nuclear transported β-catenin was significantly lower in Zic3-injected embryos. **P < 0.01, Kruskal–Wallis test followed by Steel–Dwass post hoc test. Error bars, standard error of the mean. (C) Immunoblotting analysis for β-catenin. Embryos were injected with the β-catenin or (β-catenin and Zic3) RNA. Immunoblotting of the embryo tissue lysate using an antibody against the anti-β-catenin C-terminus (β-cat.) or an antibody against the Flag-tag located on the N-terminus of β-catenin (Flag). Zic3 RNA co-injection enhanced the degradation of β-catenin. |
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ctnnb1 (catenin beta 1) gene expression in bisected Xenopus laevis embryo, NF stage 11, as assayed by in situ hybridization, lateral view, dorsal left. |
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ctnnb1 (catenin beta 1) gene expression in bisected Xenopus laevis embryo, NF stage 14, as assayed by in situ hybridization, lateral view, dorsal left. |