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Fig. 3. Spatial expression of xCTCF during Xenopus development. Whole-mount in situ hybridization was performed with xCTCF antisense RNA and staged Xenopus embryos. Nieuwkoop-Faber stages of embryogenesis are indicated. (A) Anterior view of stage 14; (B) dorsal view of stage 26; and (C) lateral view of stage 38. (C′) details as indicated by the box with broken lines in (C). (D, E) transversal vibratome sections as indicated by red dashed lines across the embryo in (C). Abbreviations: ahc, anterior hepatic cavity; ale, anterior lens epithelium; anp, anterior neural plate; ba, branchial arches; cmz, ciliary marginal zone; den, diencephalon; ev, eye vesicle; fg, frontal gland; hb, hindbrain; hm, head mesenchyme; inl, inner nuclear layer; isv, intersomitic veins; mb, midbrain; nc, notochord; np, nasal placode; nt, neural tube; ov, otic vesicle; and vz, ventricular zone. |