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Supplementary Fig. 5. Control morpholino does not affect Xotx5b and Xotx2 expression. Antibody (Ab) immunodetection (red fluorescence) of Xotx5b at st. 37 (B) and Xotx2 at st. 42 (E) in retinal sections of embryos co-injected with control morpholino and GFP as tracer (green fluorescence in A,D). (C,F) Merge. 10 nl of 125 nM control morpholino (supplied by Gene Tools) were microinjected at 4-cell stage into one dorsal blastomere. ONL, outer nuclear layer. INL, inner nuclear layer. Control morpholino injection did not affect the expression of the two proteins. |
