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Fig. 3. Dicer down-regulation delays the translation of Xotx5b and Xotx2. (A-H) Typical immunodetections (red staining) of Xotx5b (A-D) and Xotx2 (E-H) on retinal sections of morphants at different develop- mental stages, obtained in three different experiments (n > 15 embryos in each experiment). GFP (green) traces injected cells in (B,D,F,H); DAPI (blue) stains nuclei in (B,D,F,H). NT: neural tube; PE pigmented epithe- lium; ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer. The non-injected (GFP-negative) aspects of mo retinas showed normal layering, which is highlighted by dashed lines. In these parts of the retinas, Xotx5b and Xotx2 expression is comparable to that of wt retinas (Decembrini et al., 2006). Conversely, Xotx5b and Xotx2 are not detect- able in GFP-positive cells at st. 37 (A,B) and 42 (E,F), respectively. Protein detection is delayed in Mo-injected cells to later stages, namely st. 42 (Xotx5b, C,D) and st. 45 (Xotx2, G,H). Asterisk in (C) shows rosette-like structures. In (H), the Mo-injected aspect of the retina, which is GFP- labelled, shows Xotx2-positive nuclei (labelled in red). In (C), arrowheads indicate a typical monolayer of Otx5b-positive, normal photoreceptor nuclei, whereas arrows point at multilayered GFP/Xotx5b double-positive nuclei. |
