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crabp1xenopus   

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Experiment details for crabp1

Expression of key retinoic acid modulating genes suggests active regulation during development and regeneration of the amphib...

Expression of key retinoic acid modulating genes suggests active regulation during development and regeneration of the amphibian limb.

Gene Clone Species Stages Anatomy
crabp1.S laevis NF stage 56 limb , hindlimb , hindlimb digit , limb bud

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  Figure 5. Expression of Crabp1 and Crabp2 in limb development. In situ hybridisation of Crabp1 and 2 in hindlimbs (h) and forelimbs (f); dark blue stain indicates expression. A:Crabp1 is expressed weakly in the skeleton of digits I to III, as seen in the ventral view of a hindlimb at st. 56. B–J: Crabp2 expression in developing limbs. B: At st. 50, Crabp2 is expressed in the distal half of the limb bud (white asterisk) except the most distal mesenchyme cells and the epithelium. C: At st. 51, expression is limited to the central third of the mesenchyme (white asterisk). D: By st. 52, Crabp2 is expressed in patches of cells in the proximal half of the limb (black arrow), with expression also in the forming knee region (yellow arrow). E, F: Expression at the level of the knee (yellow arrow) and autopod mesenchyme (black arrowhead), in dorsal (E) and ventro-anterior (F) views of the st. 53 hindlimb. G, H: At st. 54 and 55, interdigital expression of Crabp2 (black arrowheads) is strong and there is also expression at the knee level in the anterior (yellow arrow). I, J: Dissected forelimbs showing no clear expression of Crabp2 at st. 53 and interdigital expression beginning at st. 54.