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Figure 8. Examples of embryos bisected after the in situ hybridization protocol to reveal expression patterns in internal cells. BQ399495: At blastula (left image, sagittal section), expression is primarily in the animal cap (AC) cells, and barely detected in the equatorial (eq) zone. At neural plate stages (right image, transverse section), expression is observed in the neural plate (np), somites (so), and epidermis (epi). Hprt1: At blastula (left image, sagittal section), expression is in both animal cap and equatorial cells. At gastrula stages (right image, sagittal section, anterior to the left), expression is observed in the surface ectoderm (ecto) and involuting mesoderm (meso); bl, dorsal blastoporal lip. Xxylt1: At blastula (left image, sagittal section), expression is in both animal cap and equatorial cells. At neural plate stages (right image, sagittal section, anterior to the left), expression is observed in the neural plate and underlying dorsal mesoderm (meso). IMAGE 3399268: At blastula (left image, sagittal section), expression is in both animal cap and equatorial cells. At gastrula stages (right image, sagittal section, anterior to the left), expression is observed in the animal ectoderm, but barely detected in the involuting mesoderm. Cnfn1-a: At gastrula stages (sagittal section, anterior to the left), expression is observed in the animal ectoderm, but not in the involuting mesoderm. IMAGE 7010138: At blastula (left image, sagittal section), expression is primarily in the animal cap cells, and barely detected in the equatorial zone. At gastrula stages (middle image, sagittal section, anterior to the left), there is strong expression in both the ectoderm and involuting mesoderm. At neural plate stages (right image, sagittal section, anterior to the left), weak expression is observed in both the neural plate and underlying dorsal mesoderm. |
