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FIG. 2. Expression of SGLT-1L is confined to
tubules of the pronephric kidney. SGLT-1L and
CLC-K transcripts were detected by whole-mount in
situ hybridization. Lateral views of Xenopus embryos
with anterior to the left are shown in (A-E, G,
H). Transverse sections (F, I) are oriented with dorsal
to the left. (A-D) Embryonic SGLT-1L expression
started at stage 30 (A) and remained exclusively
associated with the developing pronephric tubules
throughout tadpole stages (B-D). (E) Enlargement
of the pronephric region of the embryo shown in (D)
illustrating SGLT-1L expression in connecting tubules
(arrowheads) and the common tubule (arrow).
(F) Transverse section of stage 37/38 embryo hybridized
in whole mount for SGLT-1L expression.
Embryos were embedded in plastic and 3 μm sections
were cut at the level of the pronephros. SGLT-
1L transcripts were detected in tubular epithelia (pt),
but not in the duct (pd). (G) Double in situ hybridization
of a stage 36 embryo demonstrating SGLT-1L
(magenta) and CLC-K (blue) expression. (H) A closeup
view of the pronephric region of the embryo
shown in (G). SGLT-1L expression is present in pronephric tubules (pt), whereas CLC-K transcripts are found in the pronephric duct (pd). Arrowheads
indicate the junction between the common tubule and the duct. Selected epidermal cells express CLC-K (arrow). (I) Transverse section through the
pronephric kidney of a stage 36 embryo hybridized in whole mount for SGLT-1L and CLC-K expression. The plane of section is indicated by the dotted
line in (H). SGLT-1L and CLC-K transcripts were associated with distinct tubular (pt) and ductal (pd) epithelia, respectively. |
