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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
dll1
|
|
laevis
|
NF stage 28
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forebrain
,
midbrain
,
hindbrain
,
eye
,
late proximal tubule
,
[+]
|
wt1
|
|
laevis
|
NF stage 26
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glomerulus
,
glomus
,
pronephric kidney
|
nphs1
|
|
laevis
|
NF stage 26
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glomerulus
,
pronephric kidney
|
epha4
|
|
laevis
|
NF stage 24
|
brain
,
hindbrain
,
branchial crest
,
eye primordium
,
late proximal tubule
,
[+]
|
|
|
Fig. 6. Overexpression of Evi1-hGR in the pronephros with DEX addition from stage 18 specifically suppresses the expression of proximal but not distal markers. (A,B, E–L, O–P) Lateral views and (C, D, M, N and Q, R) sections through the pronephros of tailbud or early tadpole stage embryos coinjected with Evi1-hGR mRNA and β-galactosidase mRNA and stained with the indicated markers. Control and injected sides marked with Red-gal are shown. Note the reduction of the expression of the X-Delta-1 (A–D), EphA4 (E,F) and XSMP-30 (G, H) proximal tubule and xWT1 (K–N) and nephrin (O, R) glomus markers (arrowheads) while the expression of the Gremlin (G, H) and xClC-K (I, J) distal tubule and duct markers is unaffected (arrow). Abbreviations: pn, pronephros; spm, splanchnic layer of the mesoderm. |
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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
ret
|
|
laevis
|
NF stage 35 and 36
|
retina
,
head region
,
pronephric duct
,
pharyngeal arch
,
eye
,
[+]
|
slc26a1
|
|
laevis
|
NF stage 39
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late proximal tubule
,
early proximal tubule
,
pronephric kidney
|
pdzk1
|
|
laevis
|
NF stage 39
|
spinal cord
,
head region
,
late proximal tubule
,
early proximal tubule
,
pronephric kidney
|
|
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Fig. 3. Comparison of ClC-K, ret, Sat1, Evi1 and PDZK1 expression in Xenopus and zebrafish pronephros. The stages and probes analyzed are indicated in each figure. Anterior is toward the right. (A–D,G–J) Single in situ hybridization of the indicated markers. Note that in zebrafish ClC-K and ret1 expression is restricted to the posterior part of the duct while the proximal tubule specific markers Sat1 and PDZK1 are expressed in its anterior portion. (E) Comparison by fluorescent in situ hybridization of Evi1 (green) and ret1 (red) expression in a 24-hpf zebrafish embryo. Note that Evi1 expression extends further anteriorly than that of ret1. (F) Comparison by double in situ hybridization of ClC-K (red) and ret1 (purple) expression in a 24-hpf zebrafish embryo. Note that ClC-K expression extends further anteriorly than that of ret1. Abbreviations: apnd, anterior pronephric duct; pnd, pronephric duct; pndt, pronephric distal tubule; ppnd, posterior pronephric duct; ppt, proximal pronephric tubules. |