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Fig. 9. Blocking lif signaling in Xenopus embryo impairs normal kidney formation.
Embryos were injected co-injected unilaterally at the 8-cell stage in the prospective pronephros region with 2 ng of δlifr mRNA (δlifr) and 250 pg of lacZ mRNA as tracer control. Injected side is revealed by β-galactoside activity (red). Uninjected side is used as control (-). (A) Dorsal anterior view of a stage 42 embryo. The embryo was fixed before immunohistochemistry analysis to reveal the expression of pronephros specific marker 3G8. Arrow marks the proximal tubule. (B) Close up lateral views of representative phenotypes of embryos injected with δlifr mRNA. Histogram on the right shows the classification of 3G8 phenotypes into three categories: no effect, weak or strong effect. The total number of embryos analysed is indicated above the bars. (C) Developing embryos were fixed at stage 35–36 and analysed by in situ hybridization for wt1 expression. a, b, and c are representative phenotypes of wt1 reduced expression. Glomus region is indicated by an arrow. Histogram on the right shows the classification of phenotypes into no effect or reduced wt1 expression. The total number of embryos analysed is indicated above the bars. (D) Developing embryos were fixed at stage 35–36 and analysed by in situ hybridization for clcnkb expression. Embryos are observed on lateral view with anterior to the left and dorsal to the top. |
