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Figure 6. Xenopus golph2 is required for pronephros development. A.
Schematic diagram of the pronephros with a summary of the pronephric marker genes that were used (lateral view, anterior towards the left). At stage 38, the pronephros is subdivided into distinct segments: the glomus, proximal tubule (PT1, PT2, PT3), intermediate tubule (IT1, IT2), distal tubule (DT1, DT2) and pronephric duct (PD). B Expression of the marker genes; the orientations of panels B to M show a lateral view, the anterior towards the right; the orientations of panels Bto Mshow a lateral view, the anterior towards the left. A total of 32 ng of Control or golph2 morpholino was injected into a single ventral blastomere of 4-cell-stage embryos from the lateral region; whole-mount in situ hybridization analysis was performed on stage 38. (B Nephrin; (a) transverse section of the embryos in panels Band C (D atp1b1; (F SGLT-1K; (H ClC-K; (J NKCC2; (L NBC1. |
