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Fig. 1. xClC-K expression in the Xenopus pronephros. Twenty-five× (left) and 100× (right) images of the lateral pronephric region except where noted. (A, B) Stage 31. A faint ridge of staining in both distal tubule and duct is present, with duct staining being slightly stronger. Somite and head staining is background due to long development times. (C, D) Stage 34. Distal staining is now stronger than is duct. The red arrow indicates the point at which staining decreases. (E, F) Stage 38. (G, H) Stage 40. (I (dorsal), J) Stage 40.5. |
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Fig. 2. xClC-K expression in the distal segment and duct. All images depict transverse sections through stage 35 embryos previously stained in whole-mount for xClC-K expression with a purple substrate, then counterstained with eosin (pink). (A) Section through the pronephros. (B) Close up of panel A. Only distal tubules (DT) express xClC-K. The nephrostome (ns) and proximal tubule (PT) are not stained. (C) More posterior section through the pronephric duct. (D) Enlargement of panel C. |