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Fig. 6. The non-cell-autonomous effects of Smad7 are not mediated by the Wnt/β-catenin or the FGF pathways. When co-injected with the Wnt/β-catenin inhibitor GSK3, Smad7 still induces Sox3 (A–B), Sox2 (C–D) and Chordin (E–F). The same result is obtained when Smad7-injected embryos are grown in the presence of the FGF inhibitor SU5402: Sox3 (G), Sox2 (H) and Chordin (I) are still induced. Note that the concentration of SU5402 used is sufficient to block gastrulation and endogenous expression of these markers. |