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Display additional annotations [+]
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Figure 5. Analysis on gene expression in response to overexpression of Klf factors. A: Changes in expression of genes that are involved in germ layer formation in uninjected control embryos (ctrl) and embryos injected with mRNAs as indicated. a, animal view; l, lateral view, animal pole is up; la, lateral animal view; ld, lateral dorsal view, animal pole is up; v, vegetal view; vd, vegetal dorsal view, dorsal is up; vl, vegetal lateral view. B: Ratios (percentages) of the embryos with regular gene expression or misregulated gene expression among the total numbers (n) of control (ctrl) or injected embryos.Download figure to PowerPoint |
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Display additional annotations [+]
| Gene |
Clone |
Species |
Stages |
Anatomy |
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chrd
|
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laevis
|
NF stage 10.5
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dorsal marginal zone
,
involuting marginal zone
,
involuted dorsal mesoderm
,
upper blastopore lip
,
presumptive axial mesoderm
|
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dkk1
|
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laevis
|
NF stage 10.5
|
dorsal marginal zone
,
involuted dorsal mesoderm
,
upper blastopore lip
|
|
nodal5
|
|
laevis
|
NF stage 10.5
|
involuted dorsal mesoderm
,
upper blastopore lip
,
endomesoderm
|
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gsc
|
|
laevis
|
NF stage 10.5
|
dorsal marginal zone
,
involuting marginal zone
,
involuted dorsal mesoderm
,
upper blastopore lip
,
endomesoderm
|
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wnt8a
|
|
laevis
|
NF stage 10.5
|
lower blastopore lip
,
endoderm
,
endomesoderm
,
suprablastoporal endoderm
,
sub-blastoporal endoderm
|
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Figure 8. Analysis on the expression of genes responsible for germ layer formation in response to knockdown of Klf factors. A: Effects of knockdown of Klf factors on gene expression. The doses of each injected MO were the same as those in Figure 6B–J. All embryos are shown as vegetal view with the dorsal being placed up or as dorsal view with the animal pole being orientated up. B: Ratios of embryos with normal or disturbed transcription among the total numbers (n) of injected embryos.Download figure to PowerPoint |
