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Fig. 7. tm4sf3 mRNA rescues the morpholino knockdown phenotype. (A) Control whole gut stained for elastase expression. (B) Whole gut from embryo injected with 20 ng of morpholino. No elastase expression is detected, and the two buds did not fuse. Phenotype seen in 50/58 injected embryos. dp, dorsal pancreas; vp, ventral pancreas. The liver is outlined with a broken red line. (C) Whole gut from embryo co-injected with 20 ng tm4sf3 morpholino and 1800 pg tm4sf3 mRNA. Elastase expression is restored and the dorsal and ventral buds have fused. Rescue was seen in 59/112 injected embryos (53% rescue), 47/112 had the knockdown phenotype (42%) and 6/112 developed an annular pancreas (5%). |
