| |
Fig. 3. Nkx2.5 is necessary for differentiation of heart and myeloid cells in dorsal marginal zone (DMZ) explants. Embryos were injected
with Control morpholino oligos (MO) (18.4 pmol/embryo) (b, e, h) and with Nkx2.5 MO (18.4 pmol/embryo) (c, f, i) into the DMZ region
at the 4-cell stage. Intact embryos served as controls (a, d, g). DMZ explants excised from these embryos were cultured until st. 18 and
processed for whole-mount in situ hybridization to examine the expression of c/ebpa (a, b, c), spib (d, e, f), and mpo (g, h, i). The number
in each panel indicates total number of samples from three independent experiments. As was observed in the experiment using
whole embryos, expression of myeloid cell markers was suppressed by injection of Nkx2.5 MO (c, f, i). In contrast, expression of myeloid
cell markers was not affected by injection of Control MO (d, e, f). |
