|
Gene |
Clone |
Species |
Stages |
Anatomy |
cdc42.L
|
|
laevis
|
NF stage 11
|
ectoderm
,
neuroectoderm
,
involuted dorsal mesoderm
,
involuted ventral mesoderm
,
blastocoel roof
|
cdc42.L
|
|
laevis
|
NF stage 12
|
ectoderm
,
neuroectoderm
,
prechordal plate
,
involuted dorsal mesoderm
,
involuted ventral mesoderm
,
[+]
|
cdc42.L
|
|
laevis
|
NF stage 13
to
NF stage 15
|
ectoderm
,
epidermis
,
axial mesoderm
,
neural plate
,
paraxial mesoderm
|
cdc42.L
|
|
laevis
|
NF stage 28
|
epidermis
,
notochord
,
ventral blood island
,
pronephric mesenchyme
,
lateral plate mesoderm
,
[+]
|
|
|
FIG. 2. Expression pattern of XCdc42 in early development. (A) Temporal expression pattern of XCdc42. Developmental stages are shown above each lane. Ornithine decarboxylase (ODC) was used as a loading control. (B) Spatial expression pattern of XCdc42. (a) Sagittal section of a stage 11 gastrula. An arrow indicates dorsal lip of blastopore. (b) Higher magnified view of (a). XCdc42 transcripts are detectable in the deep cells of involuting mesoderm (arrowheads) and in the presumptive neuroectoderm (arrows). (c) Sagittal section of a stage 12 gastrula. Arrowheads indicate involuting mesoderm. (d) Higher magnified view of (c). Relatively low levels of XCdc42 expression are present in the deep layers of dorsal mesoderm (arrowheads), with its strong expression in the overlying ectoderm (arrows). (e) Transverse section of an early neurula. Strong expression of XCdc42 is present in the neural plate (NP), somitic mesoderm (S), notochord (No), and ectoderm. (f) Cross section of a tailbud stage embryo. XCdc42 is expressed in the pronephric anlage (PA), neural tube, and notochord, but not in the segmented somites (S). |