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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
actc1
|
|
laevis
|
NF stage 28
|
heart
,
cardiac mesoderm
,
heart primordium
|
isl1
|
|
laevis
|
NF stage 28
|
heart
,
cardiac mesoderm
,
heart primordium
|
tnni3
|
|
laevis
|
NF stage 28
|
heart
,
cardiac mesoderm
,
heart primordium
|
tbx20
|
|
laevis
|
NF stage 28
|
cement gland
,
heart
,
cardiac mesoderm
,
heart primordium
|
nkx2-5
|
|
laevis
|
NF stage 28
|
heart
,
cardiac mesoderm
,
heart primordium
|
wnt11
|
|
laevis
|
NF stage 28
|
mandibular crest
,
hyoid crest
,
branchial crest
,
cement gland
,
heart
,
[+]
|
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Fig. 5. Loss of DM-GRASP affects marker gene expression in the FHF but not the SHF. (A) DM-GRASP MO was unilaterally injected at the 8-cell stage and expression of cardiac marker genes was monitored at stage 20. No changes for Tbx20, Isl-1 and Nkx2.5 could be detected. Anterior views of whole embryos are given. (B) DM-GRASP MO was unilaterally injected at the 8-cell stage and expression of cardiac marker genes as indicated was monitored at stage 28. Loss of DM-GRASP resulted in down-regulation of some marker genes of the FHF (arrows) but not SHF such as Isl-1 or BMP-4. Ventral views of embryos are given. (C) Quantitative presentation of observed phenotype in panel B. Error bars are standard errors of the means. N = number of examined embryos, n = number of independent experiments. |