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Fig. 5. The expression of differentiation cardiac markers is downregulated upon loss of Dkk1 and can be rescued by co-injection of Dkk1 MO and human DKK1. A. Front view embryos of stage 28 showing reduced expression of cardiac marker genes on MO injected side (black arrow). Control MO or Dkk1 MO was injected unilaterally at the dorsal-vegetal site in embryos at stage 4. Un-injected side served as internal control. Data quantification is shown in B. C. Co-injection of Dkk1 MO and human DKK1 restore the reduced expression (black arrow) of markers as shown in stage 28 embryos of front view. Data quantification is presented in D. White dotted lines indicate the cement gland. Mean values with standard errors are given. n = number of independent experiments, N = total number of embryos analyzed. *p < 0.05, **p < 0.01 with Mann-Whitney rank sum test. |
