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Fig. 4. Induction of xFog expression by growth factors. (A) Semiquantitative RT-PCR analysis at stage 24, of xFog expression after growth factor induction in animal cap assays. Key: −, no reverse transcriptase; Ac, Activin; B, BMP-4; Sm, Xmad; Vg, AVg; F+, bFGF; F−, without growth factor; W, whole embryo. BMP- 4, Smad and Avg each induce xFog, whereas xGata-1 is induced by BMP-4 and Smad, only. ODC is the RNA loading control. (B) In whole embryos, BMP-4 signaling increases the area of xFog expression (top panel; ventralized embryos) compared to uninjected sibling controls (bottom panel). |
