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bmp4xenopus   

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Experiment details for bmp4

Structure and expression of Xenopus tropicalis BMP-2 and BMP-4 genes.

Structure and expression of Xenopus tropicalis BMP-2 and BMP-4 genes.

Gene Clone Species Stages Anatomy
bmp4 tropicalis NF stage 10.5 to NF stage 12.5 mesoderm , ventro-lateral marginal zone , lower blastopore lip
bmp4 tropicalis NF stage 21 mesoderm , head mesoderm , ventral , posterior , cardiac progenitor cell , [+]
bmp4 tropicalis NF stage 25 otic vesicle , cement gland , cardiac mesoderm , heart primordium , head

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  Fig. 3. Spatial distribution of BMP-2 and BMP-4 transcripts during embryogenesis. The spatial expression of X. tropicalis BMP-2 (A–F) and BMP-4 genes (G–L) was analysed by whole mount in situ hybridization (Harland, 1991). Digoxygenin-labelled antisense probes were derived from the translated regions of exon III, generated by PCR and subcloned into pSPT 18 (Roche). (A) morula, (B) neurula (anterior view), (C) end of neurulation, (D,E) early and late organogenesis, respectively, (F) hatching embryo, (G) midgastrula (vegetal view), (H) early neurula (posterior view), (I) end of neurulation, (J,K) early and (L) late organogenesis stages, respectively. Embryos shown in (C–F) and (I–L) are positioned with anterior to the right and posterior to the left side. All embryos have been de-pigmented in 10% H2O2/methanol and embryos shown in (I–K) are cleared in benzylbenzoate. bs, blastoporal slit; cg, cement gland; dl, dorsal lip; e, eye; h, heart; hf, heart field; hm, head mesenchyme; lpm, lateral plate mesoderm; nc, neural crest; ol, olfactory placodes; ov, otic vesicle; pg, pineal gland.