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Figure 4. Cardiac gene expression at stage 41 by in situ hybridization on section. Transverse and sagittal sections are at the level of the OFT and AV canal, respectively. A, J: Hematoxylin/eosin (H-E) stained sections. Bmp4 (B, K), Msx1 (C, L), and Nkx2.5 (D, M) are expressed in the myocardium. Frzb1 (E, N), Sox8 (F, O), Sox9 (G, P), and Xl-Fli (I, R) are expressed in the mesenchyme of the OFT and AV cushions. Sox10 (H, Q) is weakly expressed in the OFT mesenchyme. AV, atrioventricular cushion; L, liver; OFT, outflow tract cushion; V, ventricle. Scale bar = 50 mu m. |
