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atp1b3xenopus   

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Experiment details for atp1b3

Novel gene expression domains reveal early patterning of the Xenopus endoderm.

Novel gene expression domains reveal early patterning of the Xenopus endoderm.

Gene Clone Species Stages Anatomy
atp1b3.L laevis NF stage 11 to NF stage 20 notochord , blastopore lip , endoderm , neural tube

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  Fig. 5. Gastrula and neurula stage expression. In situ hybridization to gastrula and neurual stage Xenopus embryos with Endocut [darmin], Vito [slc5a8.1] , β3 [] and Fetuinish (fetub) antisense probes. The two left panels show whole mount in situ preparations to stage 11 gastrula embryos, from a vegetal view (extreme left) and a mid-sagital section, with dorsal/anterior to the left. The superficial endoderm of the blastopore lip (red arrow head) expresses Vito and β3 but not Endocut. The stage 15–18 embryos are bisected mid-sagitally and shown with anterior to the left and dorsal up. The stage 20 embryos are shown in cross section with dorsal up.

Gene Clone Species Stages Anatomy
atp1b3.L laevis NF stage 22 to NF stage 26 brain , endoderm , liver diverticulum , foregut , cloaca , [+]
atp1b3.L laevis NF stage 28 to NF stage 29 and 30 brain , endoderm , liver diverticulum , post-anal gut , cloaca , [+]
atp1b3.L laevis NF stage 35 and 36 hindbrain , spinal cord , endoderm , gall bladder primordium

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  Fig. 2. Genes expressed in the Xenopus ventral midgut from stages 22–39. In situ hybridizations with antisense Vito [slc5a8.1], β3 ATPase subunit [atp1b3], FigoB [nickipsd], Sam68 [khdrbs1], Pil [ppp1r1a] and EphrinB1 [efnb1] probes. Black arrows indicate expression in the liver diverticulum and the red arrows indicate the presumptive gall bladder. The thin black line indicates the position along the anterior-posterior axis where the cross sections on the right were taken. For the whole mount preparations anterior is to the right and in sections dorsal is up. The spot of dark staining with Vito, and Figo on the flank of the tadpole embryos is the developing pronephros.