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Fig. 3. Expression of XSGLT1k examined by colorimetric in situ, FISH and FCIS methodologies. (A and B) XSGLT1k expression in stage 28/29 pronephros (red arrow). (C and D) XSGLT1k expression in stage 37 pronephros. (E) XSGLT1K expression in stage 37 embryo detected via FISH with an FITC-tyramide substrate. (F) Na+K+ATPase expression in the same embryo as panel F, detected with a Cy3-tyramide substrate. (G) Overlay of panels E and F. The proximal domain expressing XSGLT1k appears green. (H) XSGLT1k expression in a stage 30 embryo developed with a purple substrate. (I) The same embryo as shown in panel H but developed for Na+K+ATPase expression with an FITC-tyramide substrate. (J) FCIS overlay of panels H and I performed via the protocol described in Methods. The XSGLT1k domain appears blue, while the pronephric tubules in which it is not expressed appear orange. |
