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Figure 1: Examples of the whole mount in situ hybridization on Xenopus embryos. The blue staining from an in situ hybridization experiment can clearly delineate developing structures of the early Xenopus embryo. (A) An anterior view of a stage 26 embryo highlighting the hatching gland as demarcated by the expression of uvs.2 (B) A ventral view of an embryos showing the location of early myeloid cells at stage 20 using the expression of myeloperoxidase15 as a marker. (C) The early heart at stage 28-30 is visualized by the expression of cardiac troponin I . A clear advantage of using this method is that all of these gene expression patterns were visualized using different probes but the protocol used is identical in all cases. |
