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FIG. 2. Localization of UVS.2 transcript at the neurula stage by in situ hybridization. The planes of sections corresponding to A-E are shown
in the top left diagram, a dorsal view of the embryo. The region that hybridized to the UVS.2 35S-labeled antisense RNA probe, depicted in
black, is shown in an anterior view of a stage 17 embryo (top right diagram). The micrographs show the in situ hybridization with dark-field
optics as well as the phase-contrast view of the same sections stained with Giemsa. Hybridized sections were exposed for 10 days at 4°C. Labels
indicate the neural fold (nf) and cement gland (cg). |
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FIG. 3. Localization of the UVS.2 protein using immunofluorescence.
Whole mounts of stage 36 embryos were immunostained with
an anti-peptide antibody specific for the UVS.2 protein (see Materials
and Methods). The antigen was visualized using a rhodamine-labeled
second antibody. (B, D) Specific staining of putative hatching gland
cells with the anti-UVS.2 peptide antibody. (A, C) Negative controls
in which the antibody reactions were blocked by addition of 25 rg/ml
of the UVS.2 peptide. (E) A section through the head of a stage 36
embryo that has been immunostained with the UVS.2 peptide antibody,
as seen in dark-field optics. The plane of this section is indicated
in A by the dashed line. The cells staining are noted by the arrows,
and the brain (b) and eye (e) are marked. |
