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Fig. 2. In situ hybridization of cultured explants and tailbud stage control embryos. Antisense RNA probes were digoxygenin-UTP-labeled (dark blue). (a-c) Stage 10 ventral animal caps analyzed for XCG expression; (d-f) stage 10.5 dorsal ectoderm, underlain by mesendoderm and analyzed for XCG expression; (g-i) stage 11.5 dorsal ectoderm analyzed for XCG expression; (j-l) stage 10 animal caps analyzed for XK81 expression; (m-o) stage 10 animal caps analyzed for UVS-2 expression. Control embryos, photographed at the same magnification as explants, are shown in the right column. Black arrowheads show examples of cement gland staining, white arrowhead shows hatching gland and white arrow shows XK81 staining. VE, ventral ectoderm; DE, dorsal ectoderm. The fourth column shows tailbud stage control embryos. Scale bar, 200 um. |
