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Fig. 3.
Expression patterns of the xrasgrp2 mRNA. (A) Temporal expression pattern of xrasgrp2 mRNA assessed by RT-PCR. ODC expression serves as a quantitative control. (B) Spatial expression patterns of xrasgrp2 mRNA (upper panel) and X-msr mRNA (lower panel). Whole-mount in situ hybridization was performed using stage 35 embryos. ISV, intersomitic vein; PCV, posterior cardinal vein; AA, aortic arch; VVN, vascular vitelline network. (C) Sections of the transplants were hybridized with DIG-labeled xrasgrp2 probes, which were visualized with NBT/BCIP using the Ventana Discovery system. The right panel shows a higher magnification of the transplanted region. V and D indicate ventral axis and dorsal axis, respectively. Arrowheads indicate blood vessels. |
