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Figure S5 Effects of XHMGA2 knockdown on cardiac, liver/thyroid, and endothelial marker gene expression. Whole-mount in situ hybridizations was performed at stage 34. a-c. XNkx2.5 expression in uninjected (a), MO1-injected (b), and MO2-injected embryos (c). XNkx2.5 expression was downregulated by XHMGA2 knockdown. d-f. Liver and thyroid gland development was verified by the expression of XHex in uninjected (d),
MO1-injected (e), and MO2-injected embryos (f). Expression of the XHex mRNA was attenuated in liver (arrowhead) and lost in thyroid gland (asterisk) by XHMGA2 knockdown. g-i. Vascular formation was verified by the expression of Xmsr in uninjected (g), MO1-injected (h), and MO2- injected embryos (i). Vascular patterning was perturbed (asterisks) by XHMGA2 knockdown. |
