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Fig. 3. Embryos with elevated FRG1 levels have increased angiogenesis and vascular dilation. (A–G) Stage 36 tadpoles injected with 500 pg of frg1 on one side of the embryo were analyzed for vascular abnormalities by using in situ hybridization to detect dab2 (A–D) or msr (E–G) transcripts. Abnormalities included branching of the posterior cardinal vein (black arrowhead in A), dilation of the posterior cardinal vein (black arrowheads in A,C,E), dilation of intersomitic veins (black arrows in C), and improper growth and branching of intersomitic veins (black arrows in E,G). For comparison, the uninjected sides of the embryos from A,C,E are shown in B,D,F, respectively. (H) Numbers of tadpole embryos displaying vascular abnormalities after injection with 500 pg of frg1, 1 ng of frg1 and tracer alone. The numbers above the bars indicate the total numbers of embryos analyzed. Bars, 0.5 mm. |
