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Fig. 2. Depletion of FRG1 inhibits vascular development. The mild (A) and severe (B) phenotypes of the FMO1-injected (40 ng) embryos show a reduction in vascular structures compared with the uninjected sides of the same embryos (D,E), as visualized by dab2 expression. (C,F) CMO-injected and uninjected sides of the same embryo, respectively. (G,J) FMO1-injected (40 ng) and uninjected sides of the same embryo, stained for msr. The arrowheads point to intersomitic veins. (H,I) Partial and full rescue of dab2 staining by co-injection with 40 ng of FMO1 and 500 pg of X. tropicalis frg1 mRNA. Co-injection of 40 ng of FMO1 with 1 ng of X. tropicalis frg1 mRNA was lethal. (M) The percentage of injected embryos displaying loss of dab2 and msr staining. (N) The percentage of embryos rescued by co-injection of FMO1 with frg1 mRNA. The numbers above the bars indicate the total number of embryos analyzed. Abbreviations: ps, pronephric sinus; vvn, vitelline vein network; pcv, posterior cardinal vein. Bars, 0.5 mm. |