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Fig. 5. RasGRP2 mediates VEGF-A signaling. Embryos were injected with 200 pg of β-gal mRNA (A,D), 1 ng of VEGF-A mRNA (B,E) or 1 ng of VEGF-A mRNA plus 40 ng of XRASGRP2 S-MO (C,F) into two ventral-vegetal blastomeres at the 8-cell stage. The embryos were cultured until stage 31, for whole-mount in situ hybridization analysis. (A-C) Expression patterns of globin T3. (D-F) Expression patterns of Xmsr. VEGF-A inhibits globin T3 expression in the VBI. Higher-magnification images showing the Xmsr expression patterns in the VBI region are shown (lower panels). (B) VEGF-A-mediated suppression of globin T3 expression is partially rescued by co-injection of the XRASGRP2 S-MO (C). VEGF-A induces ectopic Xmsr expression in the VBI (E). VEGF-A-induced ectopic expression of Xmsr is partially rescued by co-injection of the XRASGRP2 S-MO (F). |
