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Supporting Information Figure 2. Whole mount in situ hybridisation showing expression of Crabp2 and Raldh2 in 3-day tail regenerates for wildtype st. 50 (WT) transgenic st. 50 (N1) and refractory st. 47 (st 47) tadpoles. The animals were subjected to a heat shock at 34 for 30 min and the posterior 50% of the tail was removed 3 hr later. Daily heat shocks were used to maintain transgene expression. Black arrowheads indicate Crabp2 and Raldh2 expression in the regenerating tail blastema (A, D). There is no regeneration and no expression of either gene in N1 tail stumps (B, E) or in refractory st. tails (C, F). White arrowheads, plane of amputation; yellow arrow in A, a pigment cell (melanophore). Scale bars = 1 mm. Tadpoles are oriented with anterior to the left and dorsal uppermost. |
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aldh1a2 (aldehyde dehydrogenase 1 family, member A2) gene expression in Xenopus laevis embryo via in situ hybridization, NF stage 54, hind limb only, lateral view, dorsal view, anterior up. |
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Supporting Information Figure 1. Whole mount in situ hybridisation showing expression of Crabp2 and Raldh2 in st. 56 hindlimbs and equivalent 3-day regenerates amputated at knee level at st. 56. Arrows indicate Crabp2 and Raldh2 expression in the anterior knee (A, C). Crabp2 (B) but not Raldh2 (D) shows expression in the pseudoblastema, which will not regenerate. White arrowheads mark the plane of amputation. Scale bars = 1 mm. Limbs are oriented with proximal to the left and posterior uppermost, and viewed from the dorsal side. |
