|
Display additional annotations [+]
|
|
Fig. 2. Pairwise comparison of expression patterns of genes expressed in the anterior ectoderm at the midneurula stage.
(A-D) Whole-mount in situ hybridization with probes to transcripts of the indicated pairs of genes on the left and right halves of individual embryos as it is described in Fig. 1A. (A1-D1,A2-D2) In situ hybridization on adjacent vibratome sagittal sections of individual embryos with probes to indicated pairs of transcripts. Note that panels C1,D1,C2,D2 show results of hybridization made on two pairs of adjacent sections of the same embryo. (A3-D3,A4-D4) Enlarged images of fragments framed in panels A1-D1 and A2-D2. For abbreviations, see Fig. 1A-A4. Scale bars: 200 µm (A-D2), 40 µm (A3-D4). |
|
Display additional annotations [+]
|
|
Fig. 3. Inhibition of Ras-dva1 mRNA translation by the Ras-dva1 morpholino elicits the downregulation of Agrs and a reduction of the forebrain.
(A,C) Whole-mount in situ hybridization of midneurula control embryos with dig-labeled probes to Xag and Xagr2, respectively. Anterior view with dorsal side upward. (B,B′,D,D′) Whole-mount in situ hybridization of the Ras-dva1 MO-injected midneurula embryos with dig-labeled probes to Xag and Xagr2, respectively. Fluorescent images in panels B′ and D′ demonstrate distribution of cell clones containing the co-injected FLD fluorescent tracer. (E) The telencephalon (upper row) and whole head of the control 5-day tadpole. Dorsal view, anterior to the top. (F,F′) The telencephalon (upper row) and whole head of the 5-day tadpole developed from the embryos injected with Ras-dva1 MO into the right dorsal blastomere at the 8-cell stage. Note the reduced telencephalon and eye on the injected side. The fluorescent image in panel F demonstrates the distribution of cell clones containing the co-injected FLD fluorescent tracer. (G,G′) Rescue of the Ras-dva1 MO-induced abnormalities by the co-injection of Ras-dva1 mRNA. Note the normal telencephalon and eye on the injected side (see distribution of the injected cells in panel G′). (H,H′) Whole-mount in situ hybridization of the Ras-dva1 MO-injected midneurula embryos with dig-labeled probes to FoxG1. Note the inhibition of FoxG1 expression on the injected side. See the distribution of cell clones containing the co-injected FLD fluorescent tracer in panel H′. (I,I′) Rescue of the Ras-dva1 MO-induced inhibition of FoxG1 expression by the co-injection of Ras-dva1 mRNA. See the distribution of cell clones containing the co-injected FLD fluorescent tracer in panel I′. |
|
Display additional annotations [+]
|
|
Fig. S3. Lack of effects of control morpholino oligonucleotides on expression of all genes studied in this work. Control MO (supplementary material Table S1) to all genes whose expression was inhibited by active MO were injected in concentration 1 mM (3-5 nl/blastomere) in one of the animal dorsal blastomeres at 8 blastomere stage in mixture with living tracer FLD. Injected embryos were collected at midneurula stage and hybridized in whole-mount with probes to all genes analyzed in this work. |