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Fig. 6. Correlation between Nodal and Klf4 during embryonic development. (A) Injection of Xnr2 mRNA, dnXAR1 mRNA, Klf4 mRNA, or K4MO individually or in different combinations, as labeled above each panel, generated different effects on embryogenesis. (B) Quantification of phenotypes observed in A in three experiments. Wt, wild type; Ps, phenotypic changes after single injection; Sd, stronger phenotype after double injection; Wd, weaker phenotype after double injection; Ms, other phenotypes, such as those dead, or similar to phenotypes after single injection. (C) Expression of XAG2 in uninjected control embryos at stage 28 and injected embryos as indicated. (D) Quantification of XAG2 expression in embryos in C in four experiments. In all experiments above, 1.5 pg Xnr2 mRNA, 1.5 ng dnXAR1 mRNA, 300 pg Klf4 mRNA or 5 ng K4MO was injected. Injections of Xnr2 alone or Xnr2 plus K4MO together were radial, while others were ventral injections made at the four-cell stage. |
