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FIGURE 5.
Erlectin is a member of the endoplasmic reticulum synexpression group. The expression pattern of erlectin during Xenopus development was analyzed by RT-PCR (A) and in situ hybridization (C, E, G, I) at the indicated stages. A, expression of erlectin was analyzed by RT-PCR at the indicated stages. Histone H4 was used for normalization. C, blastula stage embryo (stage 8) showing erlectin expression in the animal hemisphere. E, during neurula stages erlectin is expressed in the notochord. The embryo was sagittally cut, anterior (a) toward the left; (p), posterior. F and G, anterior view of late neurula stage embryos showing expression of XAG and erlectin, respectively. I, tailbud stage (stage 28). The inset shows the same embryo in anterior view. H, expression of the KDEL receptor, member of the ER synexpression group (12). B and D, control hybridizations using sense riboprobe. Cg, cement gland; hg, hatching gland; no, notochord; ov, otic vesicle; pn, pronephros. |
