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Fig. 1. Knockdown ofpitx1andpitx2cinhibits endogenous cement gland formation andXag1expression. (A) 0% of uninjected embryos (n = 56) lack an observable cement gland. (B) 40% of tadpole stage embryos (n = 46) lack an observable cement gland following injection of Pitx1-MO and Pitx2c-MO. (C) 0% embryos (n = 36) lack an observable cement gland following injection of Pitx1–5MM and Pitx2c-5MM. (D) Cement gland in Pitx1-MO and Pitx2c-MO-injected embryo is rescued by injection of pitx1 RNA: 6% of embryos (n = 35) lack an observable cement gland. (E) 73% of uninjected neurula stage embryos (n = 22) exhibit strong Xag1 staining. (F) 36% of embryos (n = 28) display strong Xag1 staining following injection of Pitx1-MO and Pitx2c-MO. (G) 80% of embryos (n = 20) exhibit strong Xag1 staining following injection of Pitx1–5MM and Pitx2c-5MM. Embryos in (A-D) are lateral views, anterior to left; embryos in (E-G) are ventral views, anterior to top. Morpholino oligos were injected into the animal pole of early cleavage stages embryos. Black arrows indicate actual or expected sites of the cement gland; black arrowheads indicate Xag1 staining. |
