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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
isl1
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laevis
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NF stage 28
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heart
,
cardiac mesoderm
,
heart primordium
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bmp4
|
|
laevis
|
NF stage 28
|
heart
,
cardiac mesoderm
,
heart primordium
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tnni3
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|
laevis
|
NF stage 28
|
heart
,
cardiac mesoderm
,
heart primordium
|
tbx20
|
|
laevis
|
NF stage 28
|
cement gland
,
heart
,
cardiac mesoderm
,
heart primordium
|
nkx2-5
|
|
laevis
|
NF stage 28
|
heart
,
cardiac mesoderm
,
heart primordium
|
wnt11
|
|
laevis
|
NF stage 28
|
mandibular crest
,
hyoid crest
,
branchial crest
,
cement gland
,
heart
,
[+]
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Fig. 5. Loss of DM-GRASP affects marker gene expression in the FHF but not the SHF. (A) DM-GRASP MO was unilaterally injected at the 8-cell stage and expression of cardiac marker genes was monitored at stage 20. No changes for Tbx20, Isl-1 and Nkx2.5 could be detected. Anterior views of whole embryos are given. (B) DM-GRASP MO was unilaterally injected at the 8-cell stage and expression of cardiac marker genes as indicated was monitored at stage 28. Loss of DM-GRASP resulted in down-regulation of some marker genes of the FHF (arrows) but not SHF such as Isl-1 or BMP-4. Ventral views of embryos are given. (C) Quantitative presentation of observed phenotype in panel B. Error bars are standard errors of the means. N = number of examined embryos, n = number of independent experiments. |
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Display additional annotations [+]
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Fig. 6. ?5 -UTR DM-GRASP RNA can rescue the loss of marker gene expression in DM-GRASP MO injected embryos. (A) DM-GRASP MO was unilaterally injected at 8-cell stage together with RNA coding for ?5 UTR DM-GRASP or GFP. Expression of cardiac marker genes was monitored at stage 28. (B) Quantitative presentation of observed phenotype. Error bars are standard errors of the means. N = number of examined embryos, n = number of independent experiments.
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Display additional annotations [+]
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Fig. 7. DM-GRASP and Wnt11-R are functionally linked. (A) Wnt11-R MO was unilaterally injected at the 8-cell stage and expression of cardiac marker genes was monitored at stage 28. Wnt11-R does not affect expression of the early cardiac marker genes Nkx2.5, Isl-1, and Tbx20, but markers of differentiated cardiomyocytes of the FHF such as TnIc, cActin, and MHCα. Bar graphs depict the quantitative presentation of the observed phenotype. Error bars are standard errors of the means. N = number of examined embryos, n = number of independent experiments. (B) Co-injection of RNA coding for DM-GRASP partially reverted the phenotype triggered by the Wnt11-R MO. Bar graphs depict the quantitative presentation of observed phenotype. Error bars are standard errors of the means. N = number of examined embryos, n = number of independent experiments. |