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Figure 4. XBTBD6 knockdown affects muscle formation and segmentation. Embryos were injected on one side at the two-cell stage with either XBTBD6 MO1 or XBTBD6 MO1mis together with LacZ mRNA. Embryos were analyzed at gastrula or tadpole stage. A,B: Ventral views of embryos stained with Xbra. C,K: Lateral views of embryos stained with MyoD or c. actin. L: Horizontal section of a tail bud embryo stained with c. actin. M: Outline of muscle segmentation pattern observed in L. |