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Supp F5.
Ventral overexpression of Brg1 induces incomplete secondary body axes.
X. laevis embryos were injected in one ventral blastomere at the four cell stage with the following reagents: (A) 100pg nlacZ mRNA. The control embryo develops a normal shape. (B) 1ng brg1 mRNA results in a truncated secondary axis. (C) Frequency of 2° axes induction. * p-value ≤ 0.05. Panels (D-I) WMISH for the muscle actin gene actc1. (D-F’) Ventrally injected embryo with 100pg nlacZ mRNA in lateral (D, E) and in dorsal view (F). (F’) is a close-up of the area marked in F, showing nlacZ stained nuclei in myocytes of the second axis. Panels (G-I’) Ventrally injected Brg1 overexpressing embryo from lateral view (G, H) and dorsal view (I). (I’) shows a close-up of the area marked in (I); the arrow points to the bifurcation of primary and secondary axes, marked by nlacZ staining. |
