| |
FIG. 1. GREUL1 converts epidermis into cement gland and neural tissue in whole embryos. Embryos were injected with 500 pg of
GREUL1 mRNA into one blastomere of two-cell Xenopus embryos. At late neurula stage (19�21), the embryos were stained by in situ
hybridization for tissue-specific markers. (A, B) Laterally viewed, XAG-1-stained embryos, injected (A) compared with uninjected (B). The
inset in (B) shows an anterior view of the same embryo. (C, D) Laterally viewed, Xotx2-stained embryos, injected (C) compared with
uninjected (D). (E) Dorsally viewed, injected and Nrp1-stained embryos. The injected side, marked by an arrow, can be clearly distinguished
from the uninjected side. (F) Embryos injected at the one-cell stage with 500 pg of GREUL1 and stained for N-tubulin. (G) 500 pg
GREUL1-injected embryos stained for c-actin, showing normal somite development. In (H), the embryos were also injected with lacZ and
stained for -galactosidase activity (red) prior to in situ hybridization for slug. Dashed lines separate control and GREUL1/lacZ-injected
sides. The control side is oriented toward the top. (I�L) One-cell-stage GREUL1-injected embryos stained by in situ hybridization for both
XAG-1 (magenta) and GREUL1 (blue�green). (I) was injected with 1 ng of GREUL1, and (J, K) were injected with 500 pg of GREUL1. (L) An
uninjected control. (M) An expanded view of a portion of the embryo in (K), showing a few ectopic XAG-1 dots that appear to be outside
of the blue�green GREUL1-expressing region. |
