| |
Fig. 5. SHP-2N308D leads to alterations in cardiac myofibril formation. (A-I) Transverse sections of stage 37 hearts from uninjected (A,D,G), SHP-2FL (B,E,H) or SHP-2N308D (C,F,I) Xenopus embryos (2 ng) immunostained for fibrillin (orange) and Tmy (green) (A-C), cardiac actin as detected by conjugated phalloidin (green; D-F) and tropomyosin (red) and DAPI (blue) (G-I). Scale bar: 100 μm. (J-M) Representative transmission electron micrographs of transverse images from stage 33 (J,K) or stage 37 (L,M) heart tissue derived from uninjected (J,L) or SHP-2N308D (L,M) embryos. Cardiac muscle fibrils are highlighted. Scale bars: 1 μm. |
