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Figure 5. Loss of Xtsprouty and Xtspred Functions Causes Distinct Phenotypes(A) Cartoon depicting splice MO designs at either end of splice junctions.(B) RT-PCRs of MO-injected embryos. MO-injected lanes show bands either absent or shifted (arrowheads).(C and E) Embryos injected with MOs before first cleavage assayed for cardiac actin and FK506bp expression at stage 35 by in situ hybridization (purple). Embryos injected with 4-mis control MOs appeared identical to uninjected embryos (UI). Injection of combined Spry1 and Spry2 MOs (Spry 1+2) produced a truncated phenotype, while injection of Sprd1 and Sprd2 MOs (Sprd 1+2) produced a severe ventro-posteriorized phenotype.(D and F) Corresponding crosssections through trunks of embryos injected as above. Spry MO embryos appeared relatively normal in crosssection, but Sprd MO embryos showed disrupted somites and absence of a notochord (arrowhead). |
