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Figure 4. Expression profile of somitic markers by whole-mount in situ hybridization. Embryos are midneurula (stage [st.] 18, A,D), late neurula (st. 28, B,E,H), or early tail bud (st. 33, C,F,G,I). All images are lateral views with the exception of A and D, which are dorsal views. In all cases, anterior is to the left. A-C: Embryos stained for koza expression. D-F: Embryos assayed for myosin light chain (MLC) expression. Note the more posterior extent of MLC staining relative to the koza expression domain, particularly at midneurula. G: Double staining for both koza and alpha -cardiac actin. Areas of overlap appear as dark blue. Cells expressing only alpha -cardiac actinare light blue. Note the region of light blue staining in the posterior-most paraxial mesoderm. H,I: Embryos assayed for MHC E19 expression. Like MLC, the MHC E19 probe stains paraxial cells posterior to the koza expression domain. |
