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Experiment details for actb

Willsey HR et al. (2018) Assay

Katanin-like protein Katnal2 is required for ciliogenesis and brain development in Xenopus embryos.

Gene Clone Species Stages Anatomy
actb tropicalis NF stage 15 to NF stage 46 epidermal cell , multiciliated epidermal cell

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  Fig. 6. katnal2is required for ciliogenesis. A-J) Multiciliated X. tropicalis embryonic epidermis following injection of katnal2 morpholino at two doses or CRISPR/Cas9 RNP, showing Centrin4-CFP (blue), Actin (phalloidin, green) and acetylated α-tubulin (Ac-α-tubulin, cilia, magenta). A) Control embryos (injected with Centrin4-CFP alone) show normal ciliogenesis. B-C) Embryos injected with 2–4 pmol of katnal2 morpholino show reduced number and length of cilia. D-E) Embryos injected with katnal2 CRISPR/Cas9 RNPs also show reduced number and length of cilia. F-J) High magnification view of each condition. Asterisks (⁎) mark cells that did not undergo katnal2 disruption (Centrin4-CFP negative).

Gene Clone Species Stages Anatomy
actb tropicalis NF stage 15 to NF stage 46 multiciliated epidermal cell

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  Fig. S6. katnal2loss disrupts basal body distribution and actin network formation in theX. tropicalisembryonic epidermis. A) Control embryos showing actin (phalloidin, green), Centrin4-CFP (blue, basal bodies), and cilia (Ac-α-tubulin, magenta). B) Embryos injected with 4 pmol katnal2 morpholino show abnormal actin network formation (B), basal body distribution (B’), and cilia length and number (B”). C) Embryos injected with 6 pmol katnal2 morpholino show abnormal actin network formation (C), basal body distribution (C’), and cilia length and number (C”).

Gene Clone Species Stages Anatomy
actb.L laevis NF stage 15 to NF stage 46 epidermal cell , multiciliated epidermal cell

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  Fig. S5. katnal2loss-of-function phenotypes inX. laevis. A) Representative X. laevis embryos at stage 40 following katnal2 morpholino injection. Asterisk (⁎) indicates injected side. B) Representative X. laevis embryos at stage 44 following katnal2 morpholino injection. Asterisk (⁎) indicates injected side. C-E) Multiciliated embryonic epidermis following injection of katnal2 morpholino at two doses in X. laevis, showing Centrin4-CFP (blue), Actin (phalloidin, green) and acetylated α-tubulin (Ac-α-tubulin, cilia, magenta). C) Control embryos. D) Embryos injected with 2 pmol of katnal2 morpholino. E) Embryos injected with 4 pmol of katnal2 morpholino. Injected embryos show abnormal ciliogenesis. Asterisks (⁎) mark cells that were not targeted (Centrin4-CFP negative).