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Figure 3. CST Is Not Required for Formation or Patterning of Endodermal Tissue(A) Schematic representation of endodermal tissue markers that demarcate pharyngeal endoderm (Sox2 and Endodermin), ventral midgut (Vito and Endodermin), and posterior endoderm (Endocut).(B) Relative expression levels of endodermal markers Sox2, Vito, Endodermin, and Endocut in Stage 29 CST-depleted embryos (n = 5) relative to control MO embryos (n = 5) using GAPDH as the housekeeping gene. Bars represent the relative expression levels ± SEM.(C–F) Whole-mount in situ analysis of endodermal markers (C) Sox2, (D) Vito, (E) Endodermin, and (F) Endocut in Stage 29 (top) control MO and (bottom) CST-depleted embryos (lateral views with anterior to the left).(G–I) In situ analysis of endodermal and cardiac markers on adjacent transverse sections through the cardiac region of (top) control MO and (bottom) CST-depleted Stage 29 embryos. (G) Sox2 and Nkx2.5 expression on adjacent sections demonstrating proper expression within pharyngeal tissue of CST-depleted embryos. (H) Endodermin and Tbx20 expression on adjacent sections demonstrating proper relative spatial expression within the cardiac tissue and endoderm of the embryo. |
