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Fig. 4. Panza and Edd expression in the coiled gut and liver. In situ hybridization for Panza (A,A′,C,C′,E,E′,G,G′,I) and Edd (B,D,F,H,J) expression in guts removed from embryos at stages 40 (A,A′,B), 41 (C,C′,D), 42 (E,E′,F), 44 (G,G′,H), and 46 (I,J). Ventral (A,C,E,G) and dorsal (A′,B,C′,D,E′,F,G′,H) views with anterior left are shown. For some samples, the coiled gut was straightened before in situ hybridization and is shown with anterior left (I,J). The brown material apparent in the coiled gut shown in (J) is gut contents present before sample preparation. Arrowheads indicate the position of the liver. gb, Gallbladder; lv, liver; p, pancreas; s, stomach. |