XB-IMG-76280
Xenbase Image ID: 76280
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Fig. 4. Spatial expression pattern of XNTEF-1 and XDTEF-1 genes in developing embryos.
Whole mount in situ hybridization was performed on embryos at various developmental stages to determine the expression pattern of XNTEF-1 (A-G) and XDTEF-1 (H-N) genes. (A) In stage 17 embryo, XNTEF-1 mRNA is localized in the anterior region of the neural plate covering the eye field (arrowhead). (B) In stage 23 embryo, the expression is found in the eye (ey) and the trunk somites (s). (C) In stage 27 embryo, XNTEF-1 is strongly expressed in the brain (br), the eye (ey) and the trunk somites (s). Black line indicates the position of the section shown in (E). (D) In stage 37 embryo, XNTEF-1 is expressed in the brain, the head mesenchyme, the branchial arches (ba) and the otic vesicle (ov). There is also a widespread expression in the trunk and the posterior region. Black lines indicate the position of the sections shown in (F,G). (E) Transverse section of stage 27 embryo showing XNTEF1 expression in the ventricular zone of the midbrain (mb) and in the eye (ey). (F) Transverse section in the anterior region of stage 37 embryo showed expression in the eye (ey) and in the embryonic heart (h). (G) In transverse section of stage 37 embryo, the staining is present in the hindbrain (hb) and the otic vesicle (ov) but absent from notochord (n). (H) In stage 17 embryo, XDTEF-1 is expressed in midbrain (arrowhead) and paraxial domain (arrow). (I) In stage 27 embryo, the expression is detected in the midbrain and hindbrain regions (arrowheads), the eye (ey), the posterior somites and the presomitic mesoderm (psm). (J) In stage 33 embryo, the staining marks predominantly the forebrain (fb), the eye, the branchial arches (ba), the otic vesicle (ov) and the posterior somites (s). Black line indicates the position of the section shown in (L). (K) In stage 37 embryo, XDTEF-1 expression is found in the brain, head mesenchyme, otic vesicle (ov), trunk and posterior regions. Black lines indicate the position of the sections shown in (M,N). (L) Transverse section in the head region of stage 33 embryo showed expression in the hindbrain (hb), the otic vesicle (ov) and the branchial arches (ba). (M) Transverse section in the anterior region of stage 37 embryo showed expression in eye (ey), branchial arches (ba) and embryonic heart (h). (N) Transverse section in posterior region of stage 37 embryo showed expression in the somites (s). Image published in: Naye F et al. (2007) Copyright © 2007. Image reproduced with permission of the Publisher.
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