XB-IMG-191546
Xenbase Image ID: 191546
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Fig. 5. Tprkb expression during Xenopus development. Spatio-temporal expression pattern of tprkb visualized by whole mount in situ hybridization (WMISH). Embryonic stages and scale bars are indicated in each panel. Upper part: WMISH with exterior view. Scale bars are equivalent to 500 m. Black dashed lines represent section planes. Lower part: WMISH following sections. Scale bars are equivalent to 100 m. (A) Animal view, (B) lateral view and (C) vegetal view of Xenopus embryos in very early stages showed a very mild tprkb expression in the animal pole (arrowhead). During Xenopus embryogenesis tprkb is slightly expressed in neural tissue which is revealed by anterior view (E, F) in the anterior neural plate (anp), neural tube (nt) and the developing eye, more precisely in the eye vesicle (ev) and by dorsal view (G) in the dorsal neural tissue and neural tube (nt). (H, I, J, K) Lateral view, tprkb expression is detected in the eye vesicle (ev) or eye (e), the embryonic kidney (pronephros (p)), the otic vesicle (ov), the brain (b) and the mandibular (ma), hyoid (ha) and branchial arch (ba) which will develop into the cranial cartilage. (L) In the parasagittal view tprkb is detected in the neuroectoderm (ne). The transversal view (M, P, R) showed an expression in the mesencephalon (m), neural crest cells (ncc) and eye vesicle (ev) as well as in the eye, more detailed in the lens (le) and ciliary marginal zone (cmz). Horizontal sections (N, O, Q) revealed tprkb transcripts in the neural crest cells (ncc), mesencephalon (m), the mandibular (ma), hyoid (ha) and branchial arches (ba) and a few transcripts in the embryonic kidney (pronephric tubules (pt)). Image published in: Treimer E et al. (2021) Copyright © 2021. Image reproduced with permission of the Publisher, Elsevier B. V.
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