SLC4A4 [XNBC1]
Sodium bicarbonate cotransporter, SLC4A4. Early phase (stage 29 - 32) expression restricted to proximal segment, both early and late but stronger in early. At stage 33 a second domain of expression begins in the late distal segment. This late distal expression is much stronger than the proximal segment expression which remains on at low levels.
[click image to view enlargement]
A. |
B. |
C. |
D. |
Stage 31, lateral view.
This is the early phase of expression, which is restricted to the
proximal segment. |
Stage 35 staining. XNBC1
is shown in black, the NAK ATPase in FITC (green). |
Stage 34 embryo, close
up. Both light proximal staining and dark late distal staining is
visible. |
Stage 35. |
E. |
F. |
G. |
H. |
Stage 38. |
Stage 38-39. |
Stage 36 double stain.
This is a FCIS overlay.
XNBC1 appears blue. ATPase alone appears orange. Overlap is pink. |
Stage 38 double stain.
XNBC1 is shown in green (FITC), and XNKCC2 in red (Cy3). This is a
wholemount FISH. |
Generated by: | Original description: | ||
Agencourt: adult Xenopus laevis kidney library | Zhou, X. and Vize, P.D. (2004). Proximo-distal specialization of epithelial transport processes within the Xenopus pronephric tubules. Developmental Biology 271: 322-338. | ||
Images submitted by: | Sequence: | ||
Peter Vize | GenBank BU905206 | ||
Probe available from: | Other papers by: | ||
Peter Vize or IMAGE 4032876 | Peter Vize | ||
Probe details: | Other papers on: | ||
plasmid: pCMV SPORT6 vector | cut with: | transcribe with: | pronephroi / Xenopus cotransporters |
IMAGE 4032876 | SmaI | T7 |